Tzu Chi Medical Journal
Volume 21, Issue 2 , Pages 123-128 , June 2009

Analysis of Resistance to Clarithromycin and Virulence Markers in Helicobacter pylori Clinical Isolates from Eastern Taiwan

  • Chi-Tan Hu

      Affiliations

    • Department of Gastroenterology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
    • Department of Internal Medicine, Tzu Chi University, Hualien, Taiwan
    • ,
  • Pei-Yu Chiou

      Affiliations

    • Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
    • ,
  • Chao-Chuan Wu

      Affiliations

    • Department of Surgery, Buddhist Tzu Chi General Hospital, Taipei Branch, Taipei, Taiwan
    • ,
  • Yi-Hsiung Tseng

      Affiliations

    • Institute of Medical Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan
    • ,
  • Yao-Jen Chang

      Affiliations

    • Department of Surgery, Buddhist Tzu Chi General Hospital, Taipei Branch, Taipei, Taiwan
    • ,
  • Nien-Tsung Lin

      Affiliations

    • Institute of Microbiology, Immunology and Molecular Medicine, Tzu Chi University, Hualien, Taiwan
    • Corresponding Author InformationCorresponding author. Institute of Microbiology, Immunology and Molecular Medicine, Tzu Chi University, 701, Section 3, Chung-Yang Road, Hualien, Taiwan

Received 26 June 2008 ,Revised 25 July 2008 ,Accepted 11 August 2008.

References 

  1. Dunn BE , Cohen H , Blaser MJ . Helicobacter pylori . Clin Microbiol Rev . 1997;10:720–741
  2. Taylor DN , Parsonnet J . Epidemiology and natural history of H. pylori infections . In:  Blaser MJ ,  Smith PF ,  Ravdin J ,  Greenberg H ,  Guerrant RL editor. Infections of the Gastrointestinal Tract . New York: Raven; 1995;p. 551–564
  3. Graham DY , Lew GM , Klein PD , et al.   Effect of treatment of Helicobacter pylori infection on the long-term recurrence of gastric or duodenal ulcer. A randomized, controlled study . Ann Intern Med . 1992;116:705–708
  4. Parsonnet J , Hansen S , Rodriguez L , et al.   Helicobacter pylori infection and gastric lymphoma . N Engl J Med . 1994;330:1267–1271
  5. Forman D , Webb P , Parsonnet J . H pylori and gastric cancer . Lancet . 1994;343:243–244
  6. Parsonnet J . Gastric adenocarcinoma and Helicobacter pylori infection . West J Med . 1994;161:60
  7. Sipponen P . Helicobacter pylori and chronic gastritis: an increased risk of peptic ulcer? A review . Scand J Gastroenterol Suppl . 1991;186:6–10
  8. Hu CT , Wu CC , Lin CY , et al.   Resistance rate to antibiotics of Helicobacter pylori isolates in eastern Taiwan . J Gastroenterol Hepatol . 2007;22:720–723
  9. Versalovic J , Shortridge D , Kibler K , et al.   Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori . Antimicrob Agents Chemother . 1996;40:477–480
  10. Taylor DE , Ge Z , Purych D , Lo T , Hiratsuka K . Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations . Antimicrob Agents Chemother . 1997;41:2621–2628
  11. Occhialini A , Urdaci M , Doucet-Populaire F , Bebear CM , Lamouliatte H , Megraud F . Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes . Antimicrob Agents Chemother . 1997;41:2724–2728
  12. Stone GG , Shortridge D , Flamm RK , et al.   Identification of a 23S rRNA gene mutation in clarithromycin-resistant Helicobacter pylori . Helicobacter . 1996;1:227–228
  13. Hulten K , Gibreel A , Skold O , Engstrand L . Macrolide resistance in Helicobacter pylori: mechanism and stability in strains from clarithromycin-treated patients . Antimicrob Agents Chemother . 1997;41:2550–2553
  14. Kim KS , Kang JO , Eun CS , Han DS , Choi TY . Mutations in the 23S rRNA gene of Helicobacter pylori associated with clarithromycin resistance . J Korean Med Sci . 2002;17:599–603
  15. Toracchio S , Aceto GM , Mariani-Costantini R , Battista P , Marzio L . Identification of a novel mutation affecting domain V of the 23S rRNA gene in Helicobacter pylori . Helicobacter . 2004;9:396–399
  16. van Doorn LJ , Figueiredo C , Sanna R , et al.   Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori . Gastroenterology . 1998;115:58–66
  17. Wu CC , Chou PY , Hu CT , et al.   Clinical relevance of the vacA, iceA, cagA, and flaA genes of Helicobacter pylori strains isolated in Eastern Taiwan . J Clin Microbiol . 2005;43:2913–2915
  18. Covacci A , Censini S , Bugnoli M , et al.   Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer . Proc Natl Acad Sci USA . 1993;90:5791–5795
  19. Atherton JC , Peek RM , Tham KT , Cover TL , Blaser MJ . Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori . Gastroenterology . 1997;112:92–99
  20. Elviss NC , Owen RJ , Xerry J , Walker AM , Davies K . Helicobacter pylori antibiotic resistance patterns and genotypes in adult dyspeptic patients from a regional population in North Wales . J Antimicrob Chemother . 2004;54:435–440
  21. Yakoob J , Fan X , Hu G , Zhang Z . Genetic and phenotype changes following in vitro interactions between Helicobacter pylori strains . J Gastroenterol Hepatol . 2004;19:626–631
  22. Stege PW , Vega AE . Analysis of resistance to clarithromycin and iceA status in Helicobacter pylori isolates from San Luis, Argentina . Int J Antimicrob Agents . 2006;28:477–478
  23. Cellini L , Grande R , Di Campli E , Di Bartolomeo S , Capodicasa S , Marzio L . Analysis of genetic variability, antimicrobial susceptibility and virulence markers in Helicobacter pylori identified in Central Italy . Scand J Gastroenterol . 2006;41:280–287
  24. Akopyanz N , Bukanov NO , Westblom TU , Berg DE . PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori . Nucleic Acids Res . 1992;20:6221–6225
  25. Pena JA , Fox JG , Ferraro MJ , Versalovic J . Molecular resistance testing of Helicobacter pylori in gastric biopsies . Arch Pathol Lab Med . 2001;125:493–497
  26. Megraud F . H pylori antibiotic resistance: prevalence, importance, and advances in testing . Gut . 2004;53:1374–1384
  27. Fallone CA . Epidemiology of the antibiotic resistance of Helicobacter pylori in Canada . Can J Gastroenterol . 2000;14:879–882
  28. Osato MS , Reddy R , Reddy SG , Penland RL , Malaty HM , Graham DY . Pattern of primary resistance of Helicobacter pylori to metronidazole or clarithromycin in the United States . Arch Intern Med . 2001;161:1217–1220
  29. Mohammadi M , Doroud D , Massarrat S , Farahvash MJ . Clarithromycin resistance in Iranian H. pylori strains before introduction of clarithromycin . Helicobacter . 2003;8:80
  30. Samra Z , Shmuely H , Niv Y , et al.   Resistance of Helicobacter pylori isolated in Israel to metronidazole, clarithromycin, tetracycline, amoxicillin and cefixime . J Antimicrob Chemother . 2002;49:1023–1026
  31. Kato M , Yamaoka Y , Kim JJ , et al.   Regional differences in metronidazole resistance and increasing clarithromycin resistance among Helicobacter pylori isolates from Japan . Antimicrob Agents Chemother . 2000;44:2214–2216
  32. Yang YJ , Yang JC , Jeng YM , Chang MH , Ni YH . Prevalence and rapid identification of clarithromycin-resistant Helicobacter pylori isolates in children . Pediatr Infect Dis J . 2001;20:662–666
  33. Huang AH , Sheu BS , Yang HB , Huang CC , Wu JJ , Lin XZ . Impact of Helicobacter pylori antimicrobial resistance on the outcome of 1-week lansoprazole-based triple therapy . J Formos Med Assoc . 2000;99:704–709
  34. Ling TK , Leung WK , Lee CC , et al.   The antimicrobial susceptibility of Helicobacter pylori in Hong Kong (1997–2001) . Helicobacter . 2002;7:327–328
  35. Kim JJ , Reddy R , Lee M , et al.   Analysis of metronidazole, clarithromycin and tetracycline resistance of Helicobacter pylori isolates from Korea . J Antimicrob Chemother . 2001;47:459–461
  36. van Doorn LJ , Debets-Ossenkopp YJ , Marais A , et al.   Rapid detection, by PCR and reverse hybridization, of mutations in the Helicobacter pylori 23S rRNA gene, associated with macrolide resistance . Antimicrob Agents Chemother . 1999;43:1779–1782
  37. Hao Q , Li Y , Zhang ZJ , Liu Y , Gao H . New mutation points in 23S rRNA gene associated with Helicobacter pylori resistance to clarithromycin in northeast China . World J Gastroenterol . 2004;10:1075–1077
  38. Versalovic J , Osato MS , Spakovsky K , et al.   Point mutations in the 23S rRNA gene of Helicobacter pylori associated with different levels of clarithromycin resistance . J Antimicrob Chemother . 1997;40:283–286
  39. Stone GG , Shortridge D , Versalovic J , et al.   A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori . Antimicrob Agents Chemother . 1997;41:712–714
  40. Alarcon T , Domingo D , Prieto N , Lopez-Brea M . Clarithromycin resistance stability in Helicobacter pylori: influence of the MIC and type of mutation in the 23S rRNA . J Antimicrob Chemother . 2000;46:613–616
  41. van Doorn LJ , Glupczynski Y , Kusters JG , et al.   Accurate prediction of macrolide resistance in Helicobacter pylori by a PCR line probe assay for detection of mutations in the 23S rRNA gene: multicenter validation study . Antimicrob Agents Chemother . 2001;45:1500–1504
  42. Kato S , Fujimura S , Udagawa H , et al.   Antibiotic resistance of Helicobacter pylori strains in Japanese children . J Clin Microbiol . 2002;40:649–653
  43. Pan ZJ , Su WW , Tytgat GN , Dankert J , van der Ende A . Assessment of clarithromycin-resistant Helicobacter pylori among patients in Shanghai and Guangzhou, China, by primer-mismatch PCR . J Clin Microbiol . 2002;40:259–261
  44. Vester B , Douthwaite S . Macrolide resistance conferred by base substitutions in 23S rRNA . Antimicrob Agents Chemother . 2001;45:1–12

PII: S1016-3190(09)60023-9

doi: 10.1016/S1016-3190(09)60023-9

Tzu Chi Medical Journal
Volume 21, Issue 2 , Pages 123-128 , June 2009

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