Tzu Chi Medical Journal
Volume 21, Issue 2 , Pages 136-139, June 2009

Cystometric Changes in Pressure-guided Acute Distension Rat Model of the Underactive Bladder™

  • Dae K. Kim

      Affiliations

    • Department of Urology, Eulji University, Daejeon, Korea
    • ,
  • Jonathan Kaufman

      Affiliations

    • Lipella Pharmaceuticals, Inc., Pittsburgh, PA, USA
    • ,
  • Zhonghong Guan

      Affiliations

    • Department of Urology, State University of New York Downstate Medical School, New York, NY, USA
    • ,
  • Pradeep Tyagi

      Affiliations

    • Department of Urology, William Beaumont Hospital, Royal Oak, MI, USA
    • ,
  • Naoki Yoshimura

      Affiliations

    • Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA
    • ,
  • Kim A. Killinger

      Affiliations

    • Department of Urology, William Beaumont Hospital, Royal Oak, MI, USA
    • ,
  • Kenneth M. Peters

      Affiliations

    • Department of Urology, William Beaumont Hospital, Royal Oak, MI, USA
    • ,
  • Michael B. Chancellor

      Affiliations

    • Department of Urology, William Beaumont Hospital, Royal Oak, MI, USA
    • Corresponding Author InformationCorresponding author. Department of Urology, William Beaumont Hospital, 438, Medical Office Building, 3535 W. 13 Mile Road, Royal Oak, MI, USA

Received 19 September 2008; received in revised form 22 December 2008; accepted 26 December 2008.

Article Outline

Abstract 

Objective

Acute bladder distension results in pressure ischemia, subsequent reperfusion injury, and ultimately damage to the detrusor. We hypothesize that changes in pressure may be a key factor to damage resulting from over-distension and developed a pressure-guided distension model to evaluate cystometric changes.

Materials and Methods

Three groups of adult female Sprague Dawley rats (250 g) were used: a sham distended control group, a 3-day (3D) and 7-day (7D) follow-up group after pressure-guided distension. Under pentobarbital anesthesia, the urethra was clamped and saline was infused (0.04 mL/min) under continuous intravesical pressure monitoring. After reaching 120 cmH2O pressure, infusion was stopped and clamping was maintained for 30 minutes. For sham distension, all procedures except the saline infusion were done.

Results

There were no bladder ruptures during distension. Distension volumes needed to achieve the fixed pressure were variable (1.68–2.90 mL), but mean distension volumes were similar between the 3D and 7D groups (2.1 ± 0.1 mL vs. 2.2 ± 0.3 mL). After distension, maximal cystometric capacity and residual urine volume were increased at both time points. Voiding efficiencies were decreased significantly in both the 3D and 7D groups (p < 0.05) compared to controls. Maximal vesical pressure and bladder compliance showed no change before and after distension

Conclusion

Our pressure-guided distension model exhibits cystometric characteristics of bladder decompensation. This model for the underactive bladder™ (UAB) may prove useful to further the development of targeted UAB™ treatments.

Keywords:  Bladder , Muscle , Retention , Underactive bladder

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References 

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PII: S1016-3190(09)60025-2

doi:10.1016/S1016-3190(09)60025-2

Tzu Chi Medical Journal
Volume 21, Issue 2 , Pages 136-139, June 2009

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