Tzu Chi Medical Journal
Volume 19, Issue 4 , Pages 226-232, December 2007

Radiation Protective Effects of Cordyceps sinensis in Blood Cells

  • Chun-Chih Lin

      Affiliations

    • General Education Center, Graduate Institute of Natural Healing Sciences, Nanhua University, Chiayi, Taiwan
    • ,
  • Wilasinee Pumsanguan

      Affiliations

    • Graduate Institute of Natural Healing Sciences, Nanhua University, Chiayi, Taiwan
    • ,
  • Malcolm Mei-On Koo

      Affiliations

    • Department of Natural Biotechnology, Graduate Institute of Natural Healing Sciences, Nanhua University, Chiayi, Taiwan
    • ,
  • Hsien-Bin Huang

      Affiliations

    • Department of Life Science and Graduate Institute of Molecular Biology, Chung Cheng University, Chiayi, Taiwan
    • ,
  • Moon-Sing Lee

      Affiliations

    • Department of Radiation Oncology, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan
    • Corresponding Author InformationCorresponding author. Department of Radiation Oncology, Buddhist Dalin Tzu Chi General Hospital, 2, Min-Sheng Road, Dalin, Chiayi, Taiwan

Received 21 June 2007; received in revised form 3 July 2007; accepted 13 August 2007.

Article Outline

Abstract 

Objective

This study investigated the radiation protective effects of Cordyceps sinensis (CS) extract in C57BL/6 mice.

Materials and Methods

CS powder was extracted with methanol, concentrated and re-dissolved in de-ionized water as an extract solution. The potential of the extract to eliminate hydroxyl free radicals was measured with an ultra-weak chemiluminescence analyzer. Twelve C57BL/6 mice were used for in vivo and in vitro experiments. After exposure to 0 Gy or 5 Gy whole-body γ-ray irradiation, mice in the experimental groups (3 mice/group) were fed 1 mL of a 1000 μg/mL extract daily for 3 consecutive days while mice in the control groups (3 mice/group) were given saline. After irradiation, monocytes in the peripheral blood of the mice were separated and examined for micronuclei. Tail blood was also used for leukocyte and erythrocyte counts. Bone marrow stem cells from the mice were co-cultured with a 2 μg/mL extract for the in vitro experiment. The expression of the Cbfa (C-Module DNA-Binding Factor) gene in the experimental group was then compared with the controls after 3 days.

Results

The inhibition concentration 50% (IC50) value of CS was found to be 16.6 μg/mL, indicating that it had good potential to eliminate hydroxyl free radicals. The in vivo experiment showed that CS was able to reduce the production of micronuclei induced by radiation. The in vitro experiment indicated that Cbfa gene expression was enhanced by CS.

Conclusion

CS was able to reduce depletion in blood cells after irradiation. The radiation protective mechanisms of CS included elimination of hydroxyl free radicals generated at the initial stage of radiolysis and an increase in Cbfa expression which stimulates hematopoietic stem cell differentiation.

keywords:  Cordyceps sinensis , Free radicals , Radiation protection , Radiotherapy , Stem cells

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References 

  1. Wilson A , Trumpp A . Bone-marrow haematopoietic-stem-cell niches . Nat Rev Immunol . 2006;6:93–106
  2. Litwiejko-Pietrynczak E , Dzieciol J , Szkudlarek M . The morphological analysis of the haematopoietic micro-environment of the bone marrow at foetuses from pre-term pregnancies . Ann Univ Mariae Curie Sklodowska . 2004;59:282–285
  3. Muguruma Y , Yahata T , Miyatake H , et al.   Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment . Blood . 2006;107:1878–1887
  4. Ricci P , Tauchmanova L , Risitano AM , et al.   Imbalance of the osteoprotegerin/RANKL ratio in bone marrow micro-environment after allogeneic hemopoietic stem cell transplantation . Transplantation . 2006;82:1449–1456
  5. Ducy P , Karsenty G . Genetic control of cell differentiation in the skeleton . Curr Opin Biotl . 1998;10:614–619
  6. Gjertson C , Sturm KS , Berger CN . Hematopoietic deficiencies and core binding factor expression in murine Ts16, an animal model for Down syndrome . Clin Immunol . 1999;91:50–60
  7. Dainiak N , Sorba S . Early identification of radiation accident victims for therapy of bone marrow failure . Stem Cells . 1997;15:275–285
  8. Mundt AJ , Sibley GS , Williams S , et al.   Patterns of failure of complete responders following high-dose chemo-therapy and autologous bone marrow transplantation for metastatic breast cancer: implications for the use of adjuvant radiation therapy . Int J Radiat Oncol Biol Phys . 1994;30:151–160
  9. Rose MS . Successful repeated platelet-transfusion in radiation-induced bone-marrow failure . Lancet . 1967;1:309
  10. Lackner JM , Coad M Lou , Mertz HR , et al.   Cognitive therapy for irritable bowel syndrome is associated with reduced limbic activity, GI symptoms, and anxiety . Behav Res Ther . 1996;44:621–638
  11. Mann NS . Upper GI symptoms in patients with irritable bowel syndrome . Am J Gastroenterol . 2004;99:1191
  12. Okumura T . Tests to detect GI motility disorders and GI sensation in irritable bowel syndrome . Nippon Rinsho . 2006;64:1457–1460
  13. Wang BJ , Won SJ , Yu ZR , Su CL . Free radical scavenging and apoptotic effects of Cordyceps sinensis fractionated by supercritical carbon dioxide . Food Chem Toxicol . 2005;43:543–552
  14. Yu HM , Wang BS , Huang SC , Duh PD . Comparison of protective effects between cultured Cordyceps militaris and natural Cordyceps sinensis against oxidative damage . J Agric Food Chem . 2006;54:3132–3138
  15. Shahed AR , Kim SI , Shoskes DA . Down-regulation of apoptotic and inflammatory genes by Cordyceps sinensis extract in rat kidney following ischemia/reperfusion . Transplant Proc . 2001;33:2986–2987
  16. Lee H , Kim YJ , Kim HW , Lee DH , Sung MK , Park T . Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells . Biol Pharm Bull . 2006;29:670–674
  17. Zhang QX , Wu JY . Cordyceps sinensis mycelium extract induces human premyelocytic leukemia cell apoptosis through mitochondrion pathway . Exp Biol Med (Maywood) . 2007;232:52–57
  18. Gong HY , Wang KQ , Tang SG . Effects of Cordyceps sinensis on T lymphocyte subsets and hepatofibrosis in patients with chronic hepatitis B . Hunan Yi Ke Da Xue Xue Bao . 2000;25:248–250
  19. Nakamura K , Yamaguchi Y , Kagota S , Shinozuka K , Kunitomo M . Activation of in vivo Kupffer cell function by oral administration of Cordyceps sinensis in rats . Jpn J Pharmacol . 1999;79:505–508
  20. Koh JH , Yu KW , Suh HJ , Choi YM , Ahn TS . Activation of macrophages and the intestinal immune system by an orally administered decoction from cultured mycelia of Cordyceps sinensis . Biosci Biotechnol Biochem . 2002;66:407–411
  21. Liu WC , Wang SC , Tsai ML , et al.   Protection against radiation-induced bone marrow and intestinal injuries by Cordyceps sinensis, a Chinese herbal medicine . Radiat Res . 2006;166:900–907
  22. Winterbourn CC . Toxicity of iron and hydrogen peroxide: the Fenton reaction . Toxicol Lett . 1995;82–83:969–974
  23. Chen CW , Chiou JF , Tsai CH , et al.   Development of probe-based ultraweak chemiluminescence technique for the detection of a panel of four oxygen-derived free radicals and their applications in the assessment of radical-scavenging abilities of extracts and purified compounds from food and herbal preparations . J Agric Food Chem . 2006;54:9297–9302
  24. Hosokawa Y , Tanaka L , Kaneko M , et al.   Apoptosis induced by generated OH radicals inside cells after irradiation . Arch Histol Cytol . 2003;65:301–305
  25. Gasser S , Raulet D . The DNA damage response, immunity and cancer . Semin Cancer Biol . 2006;16:344–347
  26. Liu P , Zhu J , Huang Y , Liu C . Influence of Cordyceps sinensis (Berk.) Sacc. and rat serum containing same medicine on IL-1, IFN and TNF produced by rat Kupffer cells . Zhongguo Zhong Yao Za Zhi . 1996;21:367–369 384.
  27. Kuo YC , Tsai WJ , Shiao MS , Chen CF , Lin CY . Cordyceps sinensis as an immunomodulatory agent . Am J Chin Med . 1996;24:111–125
  28. Lee S Ka Wai , Wong C Kwok , Kong S Kai , Leung K Nam , Wai Kei Lam C . Immunomodulatory activities of HERBSnSENSES Cordyceps–in vitro and in vivo studies . Immunopharmacol Immunotoxicol . 2006;28:341–360
  29. Giuliani N , Colla S , Morandi F , Rizzoli V . The RANK/RANK ligand system is involved in interleukin-6 and interleukin-11 up-regulation by human myeloma cells in the bone marrow microenvironment . Haematologica . 2004;89:1118–1123
  30. Ribatti D , Nico B , Vacca A . Importance of the bone marrow microenvironment in inducing the angiogenic response in multiple myeloma . Oncogene . 2006;25:4257–4266
  31. Shmakova NL , Nasonova EA , Krasavin EA , Komova OV , Mel'nikova LA , Fadeeva TA . Induction of chromosome aberrations and micronuclei in human peripheral blood lymphocytes at low dose of radiation . Radiats Biol Radioecol . 2006;46:480–487
  32. Zhu JS , Halpern GM , Jones K . The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis: Part I . J Altern Complement Med . 1998;4:289–303
  33. Zhu JS , Halpern GM , Jones K . The scientific rediscovery of a precious ancient Chinese herbal regimen: Cordyceps sinensis: Part II . J Altern Complement Med . 1998;4:429–457

PII: S1016-3190(10)60020-1

doi:10.1016/S1016-3190(10)60020-1

Tzu Chi Medical Journal
Volume 19, Issue 4 , Pages 226-232, December 2007

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